Biotinylated Peptides

Custom Biotinylated Peptide Synthesis: Precision Labeling & Applications

 

Biorunstar Professional Overview: Expert Biotinylation Solutions

Biotinylation is a powerful tool for ELISA, pull-down assays, and biosensing, leveraging the ultra-strong affinity between biotin and streptavidin ($K_d \approx 10^{-14}$ mol/L). However, a successful experiment requires a precise labeling strategy to preserve biological activity.

Biorunstar Technical Excellence: Spacer & Site-Specific Optimization

To ensure the biotin-streptavidin interaction is not hindered by the peptide backbone, Biorunstar offers advanced engineering options:

Biorunstar Strategic Labeling: We offer precision N-terminal, C-terminal, or internal Lysine side-chain biotinylation to avoid interfering with the peptide's active binding site.

Biorunstar Spacer Technology: We integrate Ahx or hydrophilic PEG linkers (PEG2 to PEG12) to overcome steric hindrance and significantly improve the solubility of hydrophobic peptides.

Biorunstar Application Showcase: Scientific Research & Case Studies

Case A: Protease Biosensor (电化学传感器应用)

This research highlights a protease biosensor where Biorunstar's biotinylated peptides can be utilized as core components. By tethering the biotin-peptide to a streptavidin-covered electrode, researchers achieve high-sensitivity electrochemical analysis.

Reference: Xia, N., et al. (2021). ACS Sensors, 6(3), 1017–1024. [doi: 10.1021/acssensors.0c02408]

Case B: SARS-CoV-2 Specific Binding (病毒研究应用)

Biotinylated peptides are crucial in phage display libraries for drug discovery. This study illustrates using biotinylated peptides conjugated to streptavidin magnetic beads to identify high-affinity binders for the SARS-CoV-2 RBD.

Reference: Yang, F., et al. (2021). ACS Omega, 7 (4), 1210–1217. [doi: 10.1021/acsomega.1c05327]

Biorunstar Proven Track Record: Verified Synthesis Cases

We have retained our original synthesis case images to demonstrate our robust manufacturing capabilities

Sequence: Biotin-YGRKKRRQRRR-EXXXXXXYGXXXXXXPXXXR

Length: 31AA

M.W: 4145.89

HPLC Analysis:

MS Spectrum:

Biorunstar Quality Assurance: Each case is a testament to our ability to deliver complex sequences with verified high purity via HPLC and Mass Spectrometry (MS).

Project Documentation: Explore more structural validations at our Project Gallery.

Biorunstar Technical FAQ: Expert Insights for Researchers

Q: Why choose Biorunstar's PEG spacers over standard Ahx?

A: While Ahx is cost-effective, Biorunstar's PEG linkers provide superior flexibility and hydrophilicity, ensuring maximum biotin accessibility and better peptide solubility in aqueous buffers.

Q: Can Biorunstar combine biotinylation with other modifications?

A: Yes. We frequently synthesize multi-modified peptides involving phosphorylation, cyclization (disulfide bonds), or fluorescent labeling (FITC, Cy5) alongside biotinylation.

Q: How does Biorunstar guarantee the quality of modified peptides?

A: Every order includes a comprehensive Certificate of Analysis (COA) with HPLC and MS reports to confirm the exact molecular weight and high purity of the final product.

Partner with Biorunstar (博润思达)

For professional-grade peptide synthesis and reliable technical support, visit our official site or contact us today.

Website: www.biorunstar.com

Case Gallery: www.biorunstar.com/projects

Need a quote? Contact us: sales@biorunstar.comReceive quotation today!