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Flag Peptide, Flag peptide tag, A fusion tag known as DYKDDDDK, composed of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) and including an enterokinase-cleavage site, was specifically designed for use in immunoaffinity chromatography. This tag enables elution under non-denaturing conditions. Several antibodies targeting this peptide have been developed. One such antibody, referred to as M1, binds the peptide in the presence of divalent metal cations, preferably Ca²⁺. Elution can be achieved using chelating agents or alternatively through competitive elution with an excess of free DYKDDDDK peptide.
Specifications
|
Sequence one letter code |
DYKDDDDK |
|
Sequence three letter code |
Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys |
|
CAS Number |
98849-88-8 |
|
Molecular Formula |
C41H60N10O20 |
|
Molecular Weight |
1012.4 |
|
Modification |
N/A |
|
Purity |
Peak Area by HPLC ≥98% |
|
Form |
Lyophilized |
|
Long Storage |
- 20 °C or below |
| For Research Use Only. | |
References:
1. Einhauer A, Jungbauer A. The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 2001 Oct 30;49(1–3):455–65.
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