Biotin Peptides

What Are Biotinylated Peptides?

Biotinylated peptides are peptides that have been modified to include a biotin moiety. This modification takes advantage of the high-affinity interaction between biotin and avidin or streptavidin, which is widely used in biological and biochemical applications. The biotin molecule is small, stable, and does not significantly alter the structure or function of the peptide, while enabling highly efficient capture or detection through avidin-based systems.

 

Chemical Modification
Biotin is typically introduced through an NHS-biotin (N-hydroxysuccinimide biotin) reagent, which reacts with primary amine groups found on the peptide's N-terminus or lysine residues, forming a stable amide bond.

生物素（Biotin） 肽（Peptide）

COOH NH₂

| |

+---EDC/NHS---→ 活化羧基（Activation） → 缩合（Coupling）

↓ ↓

Biotin-CONH-Peptide

 

Affinity Interaction
The biotinylated peptide can bind strongly (Kd ~10⁻¹⁵ M) to avidin or streptavidin, making it ideal for use in assays that require precise detection, capture, or immobilization.

Biotinylated Peptide Synthesis Cases

Peptide Sequence: Biotin-Ahx-YXXXXXRXRRR-NH2

Length: 11A

Purity: >95%

M.W: 1898.32

Peptide Sequence: Biotin-YXXXXXRXRRR--ERXXXDLXXXXPVXXXXPRR

Length: 31A

Purity: >95%

M.W: 4145.89

 

Applications of Biotinylated Peptides

Biotinylated peptides are versatile tools in research and industry, with a wide range of applications, including:

Affinity Purification

Used to purify binding partners such as proteins, antibodies, or nucleic acids by immobilizing the biotinylated peptide on streptavidin beads.

ELISA and Western Blotting

Biotinylated peptides act as probes for detecting specific interactions when coupled with avidin-HRP or avidin-fluorescent labels.

Surface Plasmon Resonance (SPR)

Used for immobilizing peptides on biosensor chips for interaction studies.

Drug Discovery

Screening drug candidates by analyzing their binding to biotinylated peptide targets.

Immunology

Biotinylated peptides are used to map epitopes, study antigen-antibody interactions, and perform T-cell assays.

Epitope Mapping for Vaccine Development

A custom biotinylated peptide was used to identify antigenic epitopes of a viral protein, helping to design a vaccine candidate.

Protein-Protein Interaction Studies

Biotinylated peptides immobilized on streptavidin-coated plates enabled the study of receptor-ligand interactions, revealing binding affinities critical for drug development.

Immunoassay Optimization

In a diagnostic assay, biotinylated peptides were employed as antigens to improve the sensitivity and specificity of the ELISA for disease biomarker detection.

 

References

1. Green, N.M. (1975). Avidin and streptavidin. Biochemical Journal, 131(3), 781-791.

2. Chaiet, L., & Wolf, F.J. (1964). The properties of streptavidin, a biotin-binding protein produced by Streptomyces. Archives of Biochemistry and Biophysics, 106(1), 1-5.

3. Finn, F.M., & Titus, G. (1994). Biotinylation of proteins in research and biotechnology. Anal Biochem, 218(1), 1-7.