Custom Peptide Synthesis

Biorunstar focuses on offering high-quality custom peptide synthesis services for researchers, pharmaceutical companies, and academic institutions around the world. Our custom peptide ordering process is simple and convenient to ensure efficient order delivery.

 

Inquiry and Consultation

Send us your peptide sequence, specifications, and any additional requirements (such as purity, quantity, modifications, labeling, etc.). Our technical support team will work with you to explore ways to optimize the synthesis strategy for your project.

 

📝 Quotation and Confirmation

We will provide a detailed quotation based on your requirements. Upon your confirmation, production will begin immediately. >>🔗 How to place an order?

 

Custom Peptide Synthesis Prices

Per amino acid pricing for 6-40 mer peptides

	Crude	Desalted	>70%	>75%	>80%	>85%	>90%	>95%	>98%
1-4mg	$4.50	$5.50	$6.00	$6.50	$8.00	$9.50	$10.00	$11.00	$15.00
5mg	$5.00	$6.00	$6.50	$7.00	$8.50	$10.00	$10.50	$11.05	$17.00
10mg	$5.50	$6.50	$7.50	$8.50	$11.00	$13.00	$14.00	$16.00	$20.00
20mg	$6.00	$7.00	$9.50	$12.50	$13.50	$15.00	$16.00	$18.00	$25.00
50mg	$8.00	$9.00	$12.00	$15.50	$21.00	$25.50	$27.00	$30.00	$40.00
100mg	$10.00	$13.00	$18.00	$24.00	$30.00	$40.00	$45.00	$50.00	$60.00
500mg	$20.00	$28.00	$24.00	$40.00	$48.00	$58.00	$65.00	$70.00	$80.00
1000mg	$34.00	$42.00	$40.00	$64.00	$75.00	$80.00	$90.00	$110.00	$130.00

The prices listed on the table are estimates for standard linear peptides without modifications, special amino acids, or labeling. For a discounted and precise quotation, please contact us at sales@biorunstar.com.

Discounts are determined by factors such as peptide length, complexity, and the size of the order.

 

Our Custom Peptide Services Include

➖ Standard linear peptides

Length of sequence from 2 to 120 amino acids
Peptide quantity from lab scale to ndustrial-scale (mg -kg)

Monthly supplied capacity over 1000 peptides
Aliquots of appropriate sizes as customer requested

🔵Peptide Amidation and Peptide Acetylation

If the peptide originates from an internal protein sequence, the peptide fragment's ends in the protein would naturally be amides (CONH₂). Therefore, amidating the C-terminus and acetylating the N-terminus mimics this natural state. These modifications remove the peptide's charge and help protect it from enzymatic degradation.

Peptide Amidation.	Peptide Acetylation

🔴 Peptide synthesis modified at C terminus

C-terminus	Structure Show	C-terminus	Structure Show
Amidation		CMK/FMKChloro(fuoro)-methylketones
Aldehyde		Ester (OMe/ OEt)
Alcohols		Hydrazide
Cysteamide (Mercaptoacetamide)		OSU
AMC 7-amino-4-methylcoumarinyl		pNA (p-Nitroanilide)
AFC 7-amino-4-(trifluoromethyl)-2-benzopyrone

🔵 Peptide synthesis modified at N terminus

N-terminus	Structure show	N-terminus	Structure show
Acetylation		3-Indolylacetic acid
Benzoyl (Bz)		MpaInAA(3-Mercaptopropyl)
CBZ (Benzyloxycarbonylation)		Succinylation
Bromoacetyl (Br-Ac)		MCA6-Maleimidohexanoic acid
Formylation		Thioester
Hydroxamic acid

🧪 Other Modified Peptide Synthesis

>>🔗 Peptide Biotinylation	>>🔗 Branched peptides
>>🔗Peptide Phosphorylation	>>🔗 Methylated peptides
>>🔗 fluorescent dyes labeling	>> 🔗FRET Peptide
>>🔗 Hydrocarbon-stapled peptides	>>🔗 Stable isotope-labeled peptides
>> 🔗D-amino acid peptides	>> 🔗Chelator peptides
>>🔗 Conjugate carrier proteins	>>🔗Cys-Cys disulfide cyclization, head-to-tail cyclization

📚 Peptide Libraries Generate and Synthesis

>>🔗 Overlapping peptide library	>> 🔗Scrambled peptide library
>> 🔗Truncation peptide library	>> 🔗Alanine Scanning Library
>> 🔗T-cell truncated library	>> 🔗Positional Scanning Library

🔄 Peptide TFA desalted and transfer services

Biorunstar can provide TFA removal ( with TFA<1% guarantee) and TFA convert it to acetate or HCl salts services ( with TFA<0.1% guarantee) .

 

Custom peptides synthesized by SPPS are by default delivered as trifluoroacetic acid (TFA) salts. This is because TFA is used during the cleavage of the peptide from the solid support resin and the deprotection of amino acid side chains,Furthermore, TFA is commonly used as a component of the buffer system for HPLC purification of peptides. The TFA content typically ranges from 10% to 45%, depending on the number of Arg, Lys, and His residues in the sequence.

 

In some cellular assays, animal studies, and preclinical research cases, residual TFA may potentially cause the experiments to fail. Researchers can choose to remove the TFA or convert it to acetate or hydrochloride salts. Due to the additional TFA removal process, the cost will increases by approximately 20%. Biorunstar can provide TFA removal ( with TFA<1% guarantee) and TFA convert it to acetate or HCl salts services ( with TFA<0.1% guarantee) .

 

How To Make Peptide Synthesis

Comparison of Peptide Synthesis Methods

Method	Category	Principle	Advantages	Disadvantages	Applications
Solid-Phase Peptide Synthesis (SPPS)	Chemical synthesis	Stepwise chain elongation on solid resin using coupling-deprotection cycles (Fmoc/Boc).	Highly automated; Suitable for short peptides (≤50 AA); Compatible with modifications	Low efficiency for long peptides; Risk of truncation due to coupling failure	Lab research, peptide drug development
Liquid-Phase Peptide Synthesis (LPPS)	Chemical synthesis	Peptide chain extension or segment condensation in solution.	High intermediate purity; Scalable; Efficient for 2–6mers short peptide	Tedious steps; Less suitable for long peptides	Industrial short peptide production
Enzymatic Synthesis	Semi-chemical	Protease-catalyzed peptide bond formation.	No protecting groups; High stereoselectivity; Eco-friendly	Limited by substrate specificity; High enzyme cost	Bioactive peptide, green synthesis
Click Chemistry-Based Synthesis	Hybrid strategy	Click reactions (e.g., CuAAC) linking peptide segments.	High selectivity; Suitable for complex modifications	Requires unnatural amino acids; Complex optimization	Drug conjugates, dual-labeled probes
Recombinant Expression	Biosynthesis	Gene-based expression in host cells.	Ideal for long/structured peptides; Supports post-translational mods; Scalable	Slow; Gene construction required; Inclusion bodies possible	Vaccines, antibodies, industrial protein

Method Selection Guide

Requirement	Recommended Method	Remarks
Short peptides (<50 AA) with complex modifications	SPPS	Precise site control, high-throughput screening
Industrial-scale 2–6mer short peptide production	LPPS or SPPS	LPPS is scalable; SPPS for automation
Glycosylated or naturally folded proteins	Recombinant expression (eukaryotic)	Post-translational modification compatible
Long peptide (>50 AA) with biological activity	Recombinant expression or click chemistry	Expression is cost-effective; click suits modular design

At Biorunstar, the majority of custom peptides are synthesized by our expert chemists using state-of-the-art solid-phase synthesis techniques to ensure high purity and consistency.

Learn how peptides are synthesized using SPPS. >>🔗 What is peptide synthesis?

 

Peptide Purification and Quality Control

Why do peptides need to be purified?

Peptide purity is a critical factor that directly impacts the performance, safety, and reliability of peptide in the majority of research. To avoid interference from unwanted byproducts, At Biorunstar, we prioritize delivering high-purity peptides to ensure consistent quality and reliable results.

Common Unwanted Byproducts in Peptide Synthesis

Byproduct Type	Description
❌ Incomplete Deprotection Products	Residual protecting groups (e.g., Fmoc, Boc) not fully removed, hindering peptide function.
❌ Truncated Sequences	Peptides missing one or more amino acids due to failed coupling reactions.
❌ Deletion Sequences	Missing internal amino acids-more difficult to detect but still biologically inactive.
❌ Side-Chain Modification Products	Side reactions on reactive side chains (e.g., oxidation of Met, deamidation of Asn).
❌ Racemization Products	Formation of D-amino acids instead of L-forms, which can alter activity or reduce specificity.
❌ Isomers or Conformational Variants	Incorrect peptide folding or rearranged sequences, especially in cyclic or disulfide-linked peptides.
❌ Aggregates or Adducts	Peptide dimers, trimers, or non-specific adducts formed during synthesis or cleavage.
❌ Reagent Residues	Traces of synthesis reagents (e.g., TFA, solvents, scavengers) that remain after cleavage.

Methods for Peptide Purification

These unwanted peptide byproducts (impurities) are typically removed using one or more purification methods.

🔧 High-Performance Liquid Chromatography (HPLC)

🌊 Reverse-Phase Chromatography HPLC (RP-HPLC)

⚡ Ultra-High Performance Liquid Chromatography (UHPLC)

⚛️ Ion Exchange Chromatography (IEC)

⏳ Gel-Filtration (Size Exclusion Chromatography)

In Biorunstar's purification process, we begin with Gel-filtration for pre-treatment when necessary, which includes steps such as desalting, buffer exchange, and removal of aggregates. The most commonly used method for peptide purification is RP-HPLC (Reverse-Phase High-Performance Liquid Chromatography). This technique ensures the isolation of high-purity peptides (e.g., desalted, >75%, >85%, >95%, >98%) by effectively separating the target peptides from undesired impurities based on their hydrophobicity.  

>>🔗 Recommended custom peptide purity Levels and research applications.

 

Each peptide is subjected to strict quality control through HPLC and Mass Spectrometry (MS) analysis. A detailed Certificate of Analysis (COA) and quality report are included with every order, except for crude peptides.

 

Learn about the peptide synthesis projects we have successfully completed and delivered. >>🔗 Peptide Synthesis Cases.

 

How to Evaluate the Solubility of Your Custom Synthetic Peptide

The solubility of a peptide is determined by its physicochemical properties (hydrophobicity, charge, molecular weight) and solvent environment (polarity, pH, ionic strength). The essence of dissolution lies in disrupting intermolecular interactions (e.g., hydrophobic stacking, hydrogen bonds, salt bridges) and enhancing solvent-peptide interactions.

 

By calculating the peptide's net charge (Net Charge at pH 7), average hydrophilicity (Average Hydrophilicity), and ratio of hydrophilic residues (Ratio of Hydrophilic) using tools like theBiorunstar Peptide Calculator, the solubility difficulty in aqueous solutions can be preliminarily assessed:

⚖️Net Charge at pH 7

|Net Charge| ≥ 1: Significant charge enhances electrostatic repulsion in aqueous solutions, good solubility.

Net Charge ≥ 1: Classified as a basic peptide; use slightly acidic buffers.

Net Charge ≤ –1: Classified as an acidic peptide; use slightly alkaline buffers.

|Net Charge| < 1: Electrically neutral or weakly charged, prone to micellization or aggregation; requires organic solvents (e.g., MeOH, DMSO, DMF).

💧Average Hydrophilicity

Average Hydrophilicity ≥ 0: Overall hydrophilic tendency; good water solubility.

Average Hydrophilicity < 0: Overall hydrophobic; requires organic solvents (e.g., DMSO, DMF).

📊Ratio of Hydrophilic Residues

Ratio ≥ 50%: Soluble in water or PBS directly.

Ratio < 50%: May require organic solvents or buffers with high/low pH.

For peptides containing oxidation-sensitive residues (e.g., Cys, Met, Trp), avoid solvents or dissolution conditions that promote oxidation. such as untreated DMSO or atmospheric oxygen exposure . Use deoxygenated DMF, acidified aqueous TFA/formic acid under inert gas, and include reducing agents (e.g., DTT) when necessary.

 

Delivery and Support

Peptides are carefully packaged in lyophilized powder vials and shipped via FedEx express delivery. We also provide post-delivery support to assist with any questions or issues.

Learn about our after-sales and return policy. >>🔗 Return Policy.

 

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