Peptide nucleic acid (PNA) is an artificial nucleic acid molecule that has various advantages over natural DNA and RNA, such as high selectivity, strong affinity, and stability. Solid phase synthesis is one of the main methods for synthesizing PNA, with the following specific steps:
Selecting appropriate protecting groups: Reacting the protecting groups with the amino or carboxyl groups on the PNA precursor to form esters or amides of the protecting groups.
Connecting to polymer resin: The PNA precursor modified with a protective group is connected to the polymer resin, typically using a polymer resin with a carbon chain length of 20-30 amino acid residues.
Repeating the synthesis cycle: including removing the N-terminal protecting group; Add the next amino acid unit; Perform N-terminal protection again.
Deprotection and Separation: After synthesis, the PNA peptide chain is detached from the polymer resin through alkaline treatment or acid hydrolysis, and all protective groups are removed to obtain the PNA product.
This method can efficiently synthesize PNA molecules up to several hundred nucleotides in length, and the synthesized products have high purity, making them suitable for large-scale production
However, in practical applications, the purity of PNA aggregated by general automatic synthesizers is not ideal HiPep Research Institute has developed a novel synthetic method that can provide high-purity PNA products. This method solves some problems in traditional synthesis, such as expensive component prices, easy steric hindrance of protective groups, and significant impact of monomer purity on the biological activity of target PNA The standard synthesis range is 10-15 bases, and for synthesis of more than 16 bases, it is a special order that requires separate contact with the supplier
In addition, PNA synthesis also involves different modification methods, such as fluorescence modification, biotin modification, peptide modification, glycosylation, and DNA modification, to meet different research needs Therefore, it is very important to choose appropriate synthesis methods and techniques based on specific application scenarios and requirements.
