The molecular weight of catalogue peptides is a fundamental parameter that holds significant importance in various fields of research and pharmaceutical development. As a reputable supplier of catalogue peptides, I am well - versed in the intricacies associated with peptide molecular weights and their implications.
Understanding Peptide Molecular Weight
Peptides are short chains of amino acids linked by peptide bonds. The molecular weight of a peptide is the sum of the atomic weights of all the atoms in the peptide molecule. Each amino acid has a characteristic molecular weight, and by knowing the sequence of amino acids in a peptide, one can calculate its molecular weight. For example, glycine, the simplest amino acid, has a molecular weight of approximately 75.07 g/mol, while tryptophan, a more complex amino acid, has a molecular weight of around 204.23 g/mol.
The accurate determination of peptide molecular weight is crucial for several reasons. In research, it helps in the characterization of peptides. Scientists can use techniques such as mass spectrometry to measure the molecular weight of a peptide and confirm its identity. If the measured molecular weight deviates from the expected value, it could indicate impurities, incorrect synthesis, or post - translational modifications in the peptide.
In pharmaceutical applications, the molecular weight of a peptide can influence its pharmacokinetic properties. Smaller peptides generally have better membrane permeability and can be more easily absorbed into cells. On the other hand, larger peptides may have a longer half - life in the body but could face challenges in crossing biological membranes.
Molecular Weights of Specific Catalogue Peptides
Let's take a look at some of the catalogue peptides we offer and their molecular weights.
Cyclo(RGDfC)
Cyclo(RGDfC) is a cyclic peptide with a well - known RGD (arginine - glycine - aspartic acid) motif. This motif is recognized by integrin receptors on the cell surface, which play a crucial role in cell adhesion, migration, and angiogenesis. The molecular weight of Cyclo(RGDfC) is approximately 697.78 g/mol. This relatively small molecular weight allows it to penetrate cell membranes more readily and interact with integrin receptors effectively. It has been widely used in cancer research, as it can target tumor - associated blood vessels and inhibit angiogenesis.
Fibronectin - Binding Protein
Fibronectin - Binding Protein is a peptide that binds specifically to fibronectin, an extracellular matrix protein. Fibronectin is involved in many cellular processes, including cell adhesion, migration, and wound healing. The molecular weight of this peptide depends on its specific amino acid sequence. On average, our Fibronectin - Binding Protein has a molecular weight in the range of 2000 - 3000 g/mol. The larger molecular weight may contribute to its stability and its ability to interact with the large fibronectin molecule. It has potential applications in tissue engineering and regenerative medicine, where it can be used to promote cell adhesion and tissue formation.
TRAP - 14
TRAP - 14 is a peptide that has been implicated in various signaling pathways in the cell. It has a molecular weight of about 1600 g/mol. This peptide can be used in signal transduction research to study the role of specific signaling molecules. Its molecular weight is a key factor in its ability to interact with other proteins in the signaling cascade and modulate cellular responses.
Factors Affecting Peptide Molecular Weight
Several factors can affect the molecular weight of a peptide. One of the most obvious factors is the length of the peptide chain. As the number of amino acids in the peptide increases, so does its molecular weight. For example, a dipeptide (a peptide composed of two amino acids) will have a much lower molecular weight than a polypeptide composed of twenty or more amino acids.
Post - translational modifications also play a significant role in altering the molecular weight of a peptide. Modifications such as phosphorylation, glycosylation, and acetylation add additional chemical groups to the peptide, increasing its molecular weight. Phosphorylation, for instance, adds a phosphate group (with a molecular weight of approximately 80 g/mol) to the peptide. These modifications can also change the biological activity of the peptide, making it important to consider both the molecular weight and the modification status when using peptides in research.
The purity of the peptide can also impact the measured molecular weight. Impurities in the peptide sample, such as salts or residual solvents, can add to the overall mass and lead to inaccurate molecular weight measurements. As a supplier, we ensure that our catalogue peptides are of high purity, which helps in providing accurate molecular weight information to our customers.
Importance of Accurate Molecular Weight Information for Customers
For our customers, accurate molecular weight information is essential. In research, it is necessary for proper experimental design. For example, if a researcher is using a peptide in a cell - based assay, the correct concentration of the peptide needs to be determined based on its molecular weight. An incorrect molecular weight value could lead to inaccurate dosing, which may affect the experimental results.
In the pharmaceutical industry, accurate molecular weight information is crucial for regulatory compliance. When developing a peptide - based drug, the molecular weight of the peptide must be precisely defined and reported to regulatory agencies. Any discrepancies in the molecular weight could raise concerns about the identity, purity, and stability of the drug product.
How We Ensure Accurate Molecular Weight Determination
As a supplier of catalogue peptides, we have a rigorous quality control process in place to ensure the accuracy of the molecular weight information we provide. First, we use state - of - the - art synthesis techniques to produce peptides with the correct amino acid sequence. Our synthesis methods are optimized to minimize errors in amino acid incorporation, which can affect the molecular weight.
After synthesis, we use multiple analytical techniques to determine the molecular weight of the peptides. Mass spectrometry is our primary method for molecular weight determination. It provides high - resolution and accurate measurements of the peptide mass. We also use techniques such as high - performance liquid chromatography (HPLC) to assess the purity of the peptide and confirm that there are no significant impurities that could affect the molecular weight measurement.
Contact Us for Peptide Procurement
If you are interested in purchasing our catalogue peptides for your research or pharmaceutical development projects, we invite you to contact us. Our team of experts is available to assist you with any questions you may have regarding peptide molecular weights, applications, or ordering procedures. We strive to provide high - quality peptides with accurate molecular weight information to support your scientific endeavors. Whether you are working on a small - scale research project or a large - scale pharmaceutical development program, we can provide the peptides you need.
References
- Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., & Walter, P. (2002). Molecular Biology of the Cell. Garland Science.
- Creighton, T. E. (1993). Proteins: Structures and Molecular Properties. W. H. Freeman and Company.
- Hermanson, G. T. (2013). Bioconjugate Techniques. Academic Press.