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The 6×His tag (or Hexa-His tag) is one of the most widely used epitope tags for the purification and detection of recombinant proteins. It consists of six consecutive histidine residues and is typically added to the N- or C-terminus of proteins of interest.
Specifications
|
Sequence one letter code |
HHHHHH |
|
Sequence three letter code |
His-His-His-His-His-His |
|
CAS Number |
64134-30-1 |
|
Molecular Formula |
C36H44N18O7 |
|
Molecular Weight |
840.36 |
|
Modification |
N/A |
|
Purity |
Peak Area by HPLC ≥95% |
|
Form |
Lyophilized |
|
Long Storage |
- 20 °C or below |
| For Research Use Only. | |
References:
1. Hochuli et al., 1988: Introduced the polyhistidine tag for use in protein purification using metal affinity chromatography. 2. Porath et al., 1975: Early conceptual work on metal affinity purification methods that paved the way for His-tagging techniques.
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