Fluorescent Labeled Peptides

 

Fluorescent and dye-labeled peptides have been developed for in vivo biomedical imaging, protein binding, localization studies, and more. Fluorescent dyes can be attached directly to the N-terminus of the peptide (e.g., FITC, often with an aminohexanoic acid (Ahx) or β-Ala linker inserted between the fluorophore (fluorescein) and the peptide's N-terminus), to Lys or Cys side chains at the C-terminus, or at various other positions within the peptide.

 

Biorunstar provides many commonly used fluorescent and dye-labeled peptides, such as FITC, FAM, TAMRA, NBD (7-nitrobenz-2-oxa-1,3-diazole), Amc, Atto, Alexa Fluor, Bodipy, Cy3, Cy5, and more. Each fluorescent or dye-labeled peptide is delivered as a lyophilized powder in a foil-covered tube.

 

FITC

Fluorescein isothiocyanate (FITC), an amine-reactive derivative of fluorescein dye, has been extensively used to label peptides and proteins. It can be labeled on the peptide's N-terminus or on the lysine side chain.

Fluorescein or dye	Color	Absorption maximum	Emission maximum
FITC	Green	495nm	521nm

 

FAM

FAM is the commonly used fluorescent dye in confocal laser-scanning microscopy and flow cytometry applications.

 

Rhodamine B

Rhodamine B represents one among a numerous range of rhodamine dyes, used in fluorescence assays.

Fluorescein or dye	Color	Absorption maximum	Emission maximum
Rhodamine B	Orange	550 nm	580 nm

 

TAMRA

TAMRA is one of the most popular orange fluorophores used to label peptides and proteins.

Fluorescein or dye	Color	Absorption maximum	Emission maximum
Rhodamine B	Orange	544nm	576 nm

 

NBD (7-nitrobenz-2-oxa-1, 3-diazole)

NBD-labeled peptides is reported as a popular choice in membrane and cell biological studies.

Fluorescein or dye	Color	Absorption maximum	Emission maximum
NBD	orange	486 nm	543 nm

 

AMC

AMC (7-amino-4-methylcoumarinyl), UV-excitable dye, used in enzyme assays using cuvettes or flow cytometry.

Fluorescein or dye	Color	Absorption maximum	Emission maximum
AMC	blue	353 nm	422 nm

 

Cy3 and Cy5

Cy3 and Cy5 are dyes with extremely high extinction coefficients and fluorescence, making them especially suitable for highly sensitive localization assays of peptides in cells. However, their main disadvantage is the high instability of these molecules under peptide synthesis conditions, which leads to comparatively low yields.

 

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