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FRET Peptides
Fluorescence resonance energy transfer ( FRET) is a mechanism describing excited state energy transfer from the initially excited donor to an acceptor. The donor molecules typically emit at shorter wavelengths that overlap with the absorption of acceptors. The process is a distance-dependent...
Fluorescence resonance energy transfer ( FRET) is a mechanism describing excited state energy transfer from the initially excited donor to an acceptor. The donor molecules typically emit at shorter wavelengths that overlap with the absorption of acceptors. The process is a distance-dependent interaction between the electronic excited states of two molecules without emission of a photon. FRET peptides are widely used as suitable substrates in enzyme studies.
Biorunstar can prepare below listed Fluorophore and Quencher pairs:
|
Fluorophore |
Quencher |
Excitation |
Emission |
Comment |
|
Abz |
Dnp |
320 nm |
420 nm |
|
|
Abz |
NitroTyr |
320 nm |
420 nm |
|
|
Mca |
Dnp |
325 nm |
392 nm |
|
|
Trp |
Dnp |
280 nm |
360 nm |
|
|
EDANS |
Dabcyl 4-([4'-dimethylamino)phenyl]azo)benzoyl |
340 nm |
490 nm |
|
|
FAM |
Dabcyl 4-([4'-dimethylamino)phenyl]azo)benzoyl |
492 nm |
517 nm |
|
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