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FRET Peptides

FRET Peptides

Fluorescence resonance energy transfer ( FRET) is a mechanism describing excited state energy transfer from the initially excited donor to an acceptor. The donor molecules typically emit at shorter wavelengths that overlap with the absorption of acceptors. The process is a distance-dependent...

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Fluorescence resonance energy transfer ( FRET) is a mechanism describing excited state energy transfer from the initially excited donor to an acceptor. The donor molecules typically emit at shorter wavelengths that overlap with the absorption of acceptors. The process is a distance-dependent interaction between the electronic excited states of two molecules without emission of a photon. FRET peptides are widely used as suitable substrates in enzyme studies.

Biorunstar can prepare below listed Fluorophore and Quencher pairs:

Fluorophore

Quencher

Excitation

Emission

Comment

Abz
2-Aminobenzoyl

Dnp
2,4-dinitrophenyl

320 nm

420 nm

product-292-140

Abz
2-Aminobenzoyl

NitroTyr
3-nitro-tyrosine

320 nm

420 nm

product-339-126

Mca
7-Methoxycoumarin-4-yl)acetyl

Dnp
2,4-dinitrophenyl

325 nm

392 nm

product-383-173

Trp
Tryptophan

Dnp
2,4-dinitrophenyl

280 nm

360 nm

product-367-146

EDANS
5-[(2-Aminoethyl) amino] naphthalene-1-sulfonic acid

Dabcyl 4-([4'-dimethylamino)phenyl]azo)benzoyl

340 nm

490 nm

product-598-287

FAM
Carboxyfluorescein

Dabcyl 4-([4'-dimethylamino)phenyl]azo)benzoyl

492 nm

517 nm

product-602-232

 

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