Peptide Purity Guideline and Applications
Peptide purity has a crucial part to play in the success and integrity of research projects. Biorunstar offers a series of different purity degrees to assist customer in selecting the appropriate peptide purity for peptide-based assays and uses. Here is a list of recommended purity levels for some common peptide applications for reference.
|
Recommended Peptide Purity Levels |
Applications |
|
Desalted crude |
Epitope mapping Protein to protein interactions Immunogenic identification Receptor-ligand interactions Nucleic acid binding Mutation Screening Sequence optimization |
|
>75% by HPLC |
ELISA testing Peptide arrays Antigens for polyclonal antibody production or affinity purification |
|
>85% by HPLC |
In-vitro bioassays Epitope mapping Polyclonal antibody production Phosphorylation studies Cell attachment studies Protein electrophoresis applications Semi-quantitative enzyme-substrate studies Peptide blocking studies for Western Blot (non-quantitative) Coupling to chromatography resins for affinity purifications |
|
>95% by HPLC |
In-vitro bioassays and studies NMR studies Enzymology Quantitative receptor- ligand interactions studies Quantitative blocking and competitive inhibition studies Quantitative phosphorylation studies Quantitative proteolysis studies |
|
>98% by HPLC |
Sensitive Bioassay Cosmetic peptides Active pharmaceutical ingredients Clinical trials Crystallography SAR studies |
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