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HRP-Conjugated Secondary Antibody Peptide (IgG Universal)
Product Name: HRP-Conjugated Secondary Antibody Peptide (IgG Universal) Cross-Reactivity: Multiple species IgG (mouse / rabbit / goat / donkey, etc.) Specification: Total volume: 0.5 mL (0.3 mL labeled stock + 0.2 mL 100% glycerol) (prediluted version) Antibody Concentration: 15 µg / 0.5...
Product Name:
HRP-Conjugated Secondary Antibody Peptide (IgG Universal)
Cross-Reactivity:
Multiple species IgG (mouse / rabbit / goat / donkey, etc.)
Specification:
Total volume: 0.5 mL (0.3 mL labeled stock + 0.2 mL 100% glycerol) (prediluted version)
Antibody Concentration:
15 µg / 0.5 mL
Recommended Dilution Range:
WB (ECL detection): 1:1000 – 1:7000
WB (colorimetric detection): 1:1000 – 1:4000
Incubation Time:
15–60 min (typically 30 min)
Diluent / Blocking Buffer:
PBS buffer, pH 7.4, 0.5% BSA (no IgG, no protein)
Storage & Stability:
Store at -20°C for up to 1 year, avoid repeated freeze-thaw cycles; short-term storage at 4°C up to 2 weeks
Notes / References for Use:
|
Parameter |
Recommended Setting |
Adjustment if Needed |
|
Dilution (ECL) |
1:3000, 30 min |
Adjust within 1:1000 – 1:7000 based on signal/background |
|
Incubation Time |
Room temp, 30 min |
15–60 min, adjust according to dilution and reaction conditions |
|
Blocking |
BSA without IgG or boiled inactivated milk powder |
Avoid IgG in antibody diluent |
Note: For research use only. Not for clinical or diagnostic use.
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